Adipogen
anti-APRIL (mouse), mAb (blocking) (Centotto-1) (preservative free)
anti-APRIL (mouse), mAb (blocking) (Centotto-1) (preservative free)
APRIL is a secreted cytokine, belonging to the TNF superfamily that binds to transmembrane activator and CAML interactor (TACI), B cell maturation antigen (BCMA) and can interact with carbohydrate side chains of heparan sulfate proteoglycans (HSPG) that may trigger cross-linking. APRIL maintains B cell homeostasis by acting at a later stage, modulating the function and survival of antigen-experienced B cells. APRIL (as well as BAFF) stimulates class-switch recombination (CSR), hence contributes to shaping humoral effector mechanisms. With regards to humoral memory, APRIL is involved in the establishment and survival of the long-lived plasma cell (LLPC) pool in the bone marrow (BM). Recently, APRIL has been shown to confer atheroprotection via binding to heparan sulfate (HS) chains of heparan-sulfate proteoglycan 2 (HSPG2), which limits LDL retention,macrophage accumulation and necrotic core formation.
Product References:
1.APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans: D. Tsiantoulas, et al.; Nature 597, 92 (2021)
2.BAFF, but not APRIL, initiates Chronic Lymphocytic Leukemia by inducing tumor-promoting genes rather than cell survival: F. Mackay, et al.; ahead of print (2023)
3.An unappreciated cell survival-independent role for BAFF initiating chronic lymphocytic leukemia: M.A. Ullah, et al.; Front. Immunol. 15, 1345515 (2024)
4.Unique and redundant roles of mouse BCMA, TACI, BAFF, APRIL and IL-6 in supporting antibody-producing cells in different tissues: M. Eslami, et al.; PNAS 21, e2404309121 (2024)
Couldn't load pickup availability
General Specifications
1.Synonyms: A Proliferation Inducing Ligand; Tumor Necrosis Factor Ligand 2.Superfamily Member 13; TNFSF13; TALL-2; CD2563.Product Type: Monoclonal Antibody
Properties
1.Clone: Centotto-1
2.Isotype: Mouse IgG1κ
3.Source/Host: Purified from concentrated hybridoma tissue culture supernatant.
4.Immunogen/Antigen: Recombinant mouse APRIL.
5.Label/Conjugates: Preservative Free
6.Application:
Functional Application: Depletion of mouse APRIL.
Immunoprecipitation: (1:200)
7.Crossreactivity: Mouse
8.Specificity: Recognizes mouse recombinant and endogenous APRIL.
9.Purity: ≥95% (SDS-PAGE)
10.Purity Detail: Protein G-affinity purified.
11.Endotoxin Content: <0.01EU/μg purified protein (LAL test).
12.Concentration: 1mg/ml
12.Formulation: Liquid. In PBS.
13.Isotype Negative Control: Mouse IgG1 Isotype Control (preservative free)
14.Other Product Data:
Note: BAFF and APRIL contribute to maintaining IgG, IgA, and IgM plasma cells in the bone marrow (BM), spleen, and intestine and rely mainly on three ligand/receptor pairs: APRIL-BCMA, BAFF-TACI, and APRIL-TACI. Together, these actors account for 90% of circulating antibodies. To efficiently target plasma cells, dual inhibition of BAFF (using blocking anti-BAFF (mouse), mAb (Sandy-2) (Prod. No. AG-20B-0063)) and APRIL (using blocking anti-APRIL (mouse), mAb (blocking) (Centotto-1) (Prod. No. AG-20B-0083PF) both developed at AdipoGen Life Sciences are required in a recently published study (see also Figure).
15.Accession Number: Q9D777
16.Shipping: BLUE ICE
17.Short Term Storage: +4°C
18.Long Term Storage: -20°C
19.Handling Advice:
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
20.Use/Stability: Stable for at least 1 year after receipt when stored at -20°C.
Safety & Documentation
View full details
Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.