Adipogen
20S Constitutive Proteasome Assay Kit
20S Constitutive Proteasome Assay Kit
South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits β1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of β5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.
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General Specifications
1. Specificity: Readout: Endpoint/KineticLabel or Dye: 7-Amino-4-methylcoumarin
Detection Method: Fluorescence
Substrate Properties: Protein-based Substrate.
Excitation/Emission (nm): 345/445
2. Quantity: Sufficient Reagents for:
100 x 50 μl reactions
3. Detection Type: Fluorescent
4. Kit Contains:
10x 20S Proteasome
50x LLVY-AMC, Chemotrypsin-like activity
50x WLA-AMC, β5/PSMB5 specific substrate
50x LLE-AMC, β1/PSMB6 specific substrate
50x Inhibitor (MG-132, 2mM) in 100% DMSO
10x Reaction Buffer
50x SDS(1.75%) in H20
100x free AMC Standard (40μM)
5. Declaration: Manufactured by South Bay Bio.
6. Shipping: DRY ICE
7. Short Term Storage: -20°C
8. Long Term Storage: -80°C
Safety & Documentation
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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.