Adipogen
20S Immunoproteasome Assay Kit
20S Immunoproteasome Assay Kit
South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits β1i/ PSMB9 (PAL-AMC) and β5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit β5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
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General Specifications
1. Specificity: Readout: Endpoint/KineticLabel or Dye: 7-Amino-4-methylcoumarin
Detection Method: Fluorescence
Substrate Properties: Protein-based Substrate.
Excitation/Emission (nm): 345/445
2. Quantity: Sufficient Reagents for:
100 x 50 μl reactions
3. Detection Type: Fluorescent
4. Kit Contains: 10x 20S Immunoproteasome
50x LLVY-AMC, Chemotrypsin-like activity
50x PAL-AMC, β1i/PSMB9 specific substrate
50x ANW-AMC, β5i/PSMB8 specific substrate
50x Inhibitor (ONX-0914, 2mM) in 100% DMSO
10x Reaction Buffer
50x SDS(1.75%) in H20
100x free AMC Standard (40μM)
5. Declaration: Manufactured by South Bay Bio.
6. Shipping: DRY ICE
7. Short Term Storage: -20°C
8. Long Term Storage: -80°C
Safety & Documentation
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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.