Minute TM Total Protein Extraction Kit for Plant Tissues

The MinuteTM total protein extraction kit for plant tissues comprises an optimized protein extraction buffer and protein extraction filter cartridges accompanied by 2.0 ml collection tubes. It aims to efficiently extract denatured or native proteins from various plant tissues, including leaves, seeds, soft stems, and roots. Notably, the protein profiles obtained through denaturing and native cell lysis buffers differ. Therefore, depending on the specific application, one buffer may outperform the other. This kit offers both denaturing and native cell lysis buffers, allowing users to evaluate and select the most suitable option for their particular needs. Utilizing the protein extraction filter cartridges makes it possible to extract total plant soluble proteins from 50-200 mg of plant tissue within 5-8 minutes while achieving a high protein yield ranging from 2-8 mg/ml. This extraction method is useful for SDS-PAGE, immunoblotting, ELISA, IP, and enzyme assays. These extracted proteins can also be excellent starting materials for small-scale protein purification using column chromatography techniques.