MinuteTM Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes

Adipose tissue, especially white adipose tissue (WAT), has been recognized as an essential endocrine and inflammation organ in addition to its energy storage function. Analyses of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions. However, isolation of WAT and brown adipose tissue (BAT) is technically challenging due to their high lipid and low protein contents. The water-oil emulsion present in a biological sample is notoriously difficult to separate. We have developed a novel technology to address this issue. A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effectively separate water-oil emulsion derived from adipose tissue homogenate. The extraction buffer has a lower freezing point than oil in adipose tissues, and the aqueous phase can be quickly separated from the oil phase by passing the tissue homogenate through the filter. The total proteins isolated are the unbiased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg/ml) compared to traditional methods.