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Chemicals

Deoxyribonuclease I (DNase I)

Deoxyribonuclease I (DNase I)

SKU: PC0704-25mg
Brand: Vivantis
Lead Time: 1-2 weeks

DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA.

DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.

Mol. Formula: N/A;
Mol. Weight: 31,000 Daltons

Regular price $254.00 SGD
Regular price Sale price $254.00 SGD
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General Specifications

1. Grade: Molecular Biology Grade
2. Form: Powder
3. Appearance: White to off-white crysyalline powder
4. Storage Temperature: Store at -20°C.

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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.