Ribonuclease A (RNase A), High Purity Grade

RNase A is an endoribonuclease that is from bovine pancreas for molecular biology applications. The major application for RNase A is the removal of RNA from preparation of plasmid DNA as well as extraction of plasmid DNA. It is also used in removal of unspecifically bound RNA; RNase protection assays; analysis of RNA sequences as well as hydrolysis of RNA contained in protein samples.

RNase A attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.

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