ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, EvaGreen® dye, as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.