ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, SYBR® Green dye as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. SYBR® Green dye emits fluorescence when bound to doublestranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.

ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.