Molecular Biology
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye)
ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye)
ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I is the improved version of master mix working for one-step amplification of any RNA template including total RNA, mRNA and etc using real-time PCR method.
ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I is improved to have 4X concentration and consists of improved technology of enzyme of Reverse Transcriptase and Hot Start Taq DNA Polymerases; dNTPs/dUTP Mix, RNase Inhibitor and Heat-labile UDG that allow less contaminated reaction preparation as well as leads to increased performance in sensitivity and specificity. This master mix is having SYBR® Green dye emits fluorescence when bound to doublestranded DNA without probes. Detection of PCR product is monitored by the increase in fluorescence.
Separated tubes of ROX™ dye are provided to allow the master mixes to use with most of the real-time PCR instruments. The master mixes can be used for fast cycling as suggested for any qualitative tests.
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General Specifications
1. Features:• One step reaction set up for real-time PCR or conventional PCR
• Equipped with improved generation of thermostable Reverse Transcriptase and Hot Start Taq DNA Polymerases
• Includes SYBR® Green dye for intercalator-based qPCR
• Optimal performance for highly sensitive and specific RT-PCR reaction compared to QLMM04
• Compatible with most of the real-time PCR platforms
2. Applications: All kinds of RNA sample material suited for RT-qPCR amplification can be used.
3. Storage Temperature: Store at -20°C
Safety & Documentation
View full detailsDisclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.
