ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with Low ROX

ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix II is next generation first choice mix designed for fast and easy one step real-time PCR reaction set up. The improved formulation of master mix contains unique thermostable M-MULV enzyme, Hot Start Taq DNA Polymerases, EvaGreen® dye, as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and higher affinity for primer template duplexes which allows very rapid processing during RT step. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.

ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix II can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of RT-qPCR master mix can detect extremely low copy number targets very specifically with high efficiency that gives CT values close to the theoretical time of detection. The ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix II is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and non-specific products is prevented leading to optimum sensitivity and specificity.

ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.