ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I

ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I is next generation first choice mix designed for fast and easy one step real-time PCR reaction set up. The improved formulation of master mix contains unique thermostable MMULV enzyme, Hot Start Taq DNA Polymerases, SYBR® Green dye as well as MgCl2 and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and higher affinity for primer template duplexes which allows very rapid processing during RT step. SYBR® Green dye emits fluorescence when bound to double-stranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.

ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of RT-qPCR master mix can detect extremely low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I is a complete system for use in one step realtime PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and nonspecific products is prevented leading to optimum sensitivity and specificity.

ViPrimePLUS One Step AtTaq qRT-PCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.