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Molecular Biology

Viva cDNA Synthesis Kit

Viva cDNA Synthesis Kit

SKU: cDSK01-050
Brand: Vivantis
Lead Time: 1-2 weeks

Viva cDNA Synthesis Kit is specially designed to provide reliable synthesis of full-length cDNA. M-MuLV RNase H- synthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. This cDNA synthesis kit is readily compatible with various cDNA-dependent downstream applications.

$197.00 SGD
$197.00 SGD
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Pack Size

General Specifications

1. Kit Component:
• 5,000u M-MuLV Reverse Transcriptase
• 125µl 10X Buffer M-MuLV
• 125µl 10mM dNTPs mix
• 50µl Oligo d(T)18 (40µm)
• 50µl Random hexamer (50ng/µl)
• 1ml Nuclease-free water
2. Features:
• Absence of RNase H activity allows high yield of full length cDNA synthesis with RNA templates up to 10kb
• Wide selection of primers, oligo d(T) or random hexamer
• Highly compatible with various downstream applications
• Allows synthesis of full length cDNA from various RNA templates
• High capacity and able to copy up to 2µg of purified mRNA
3. Applications:
• Traditional PCR amplification or real-time PCR quantification
• Storage of selective and functional genetic information by removing of introns
• Cloning of the gene of interested enables horizontal gene transfer (HGT)
• cDNA probe library construction with large and full-length inserts
• cDNA microarray for gene discovery and clinical diagnosis via gene pattern screening
• mRNA expression studies and storage of proteins material at genomics level
4. Storage Temperature: Store at -20°C

Safety & Documentation

MSDS
Manual Viva cDNA Synthesis Kit

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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.