Molecular Biology
Chromo Taq DNA Polymerase (1U/µl)
Chromo Taq DNA Polymerase (1U/µl)
SKU: PL1205
Brand: Vivantis
Lead Time: 2-4 weeks
Chromo Taq DNA Polymerase is a thermostable DNA polymerase. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymerase catalyzed the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of MgCl2 and has the 5' to 3' exonuclease activity. The enzyme is supplemented with inert color tracer dyes.
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General Specifications
1. Supplied With:• 10X ViBuffer A (without MgCl2)
500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP
• 10X ViBuffer S
160mM (NH4)2SO4, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
• 50mM MgCl2
2. Features:
• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 20kb.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.
• Generates mostly 3' dA overhang amplification products which are suitable for TA cloning.
3. Storage Temperature: Store at -20°C
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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.