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Molecular Biology
2X Pfu Master Mix
2X Pfu Master Mix
SKU: PLMM03
Brand: Vivantis
Lead Time: 1-2 weeks
2X Pfu Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture containing Pfu DNA Polymerase, reaction buffer, dNTPs and enhancers. It contains all the components required for routine DNA amplification except template and primers. Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase. It exhibits the 3’ to 5’ proofreading activity, resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerases.
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General Specifications
1. Supplied with:• 4 x 625µl 2X Pfu Master Mix*
• 3ml of Nuclease-free Water
*2X Pfu Master Mix consists of Pfu DNA Polymerase, (2X ViBuffer Pfu, 0.4mM dNTPs and enhancers)
2. Features:
• Saves time and reduces contamination due to reduced number of pipetting steps.
• Recommended for use in high-fidelity amplification, amplification of GC-rich sequences or problematic secondary structures, primer extension reactions at elevated temperatures and cloning of blunt-ended amplification products.
• Stable at 4˚C for 6 months, allowing immediate reaction setup without the time-consuming thawing of reagent.
• Suitable for all routine DNA amplification applications.
3. Storage & Stability:
• Stable at -20°C for 18 months or at 4°C for 6 months if properly stored.
• Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
• For daily use, keeping aliquots at 4°C is recommended.
Safety & Documentation
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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.