Molecular Biology
T4 DNA Ligase
T4 DNA Ligase
SKU: ME4303
Brand: Vivantis
Lead Time: 1-2 weeks
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA-RNA hybrids.
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$81.00 SGD
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General Specifications
1. Concentrations: 50 - 200u/μl2. Supplied With:
10X Buffer T4 Ligase
50mM Tris-HCl (pH7.8 at 25˚C), 10mM MgCl2, 10mM DTT, 1mM ATP and 25μg/ml BSA. Store at -20˚C.
3. Application:
• Catalyzes the linkage of 5' or 3' blunt/cohesive ends of double-stranded DNA by formation of phosphodiester bond.
• Joining of oligonucleotide linkers or adapters to blunt ends.
• Repair nicks formation in duplex nucleic acids.
4. Storage Temperature: Store at -20°C.
Safety & Documentation
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Disclaimer: Products are chemical reagents for research use
only and are not intended for human use. We do not sell to patients.