Human RECK (Reversion-inducing cysteine-rich protein with Kazal motifs) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti RECK antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with RECK bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of RECK in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alias: Reversion-inducing cysteine-rich protein with Kazal motifs ELISA Kit, hRECK ELISA Kit, Suppressor of tumorigenicity 15 protein ELISA Kit, RECK ELISA Kit, ST15 ELISA Kit
Species: Human
Range: 0.625-40ng/ml
Sensitivity: 0.375ng/ml
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Storage: 2-8°C(Sealed), Don't cryopreserve
Specificity: Specifically binds with RECK , no obvious cross reaction with other analogues