Rat MDA (Malonaldehyde) ELISA Kit

This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with MDA. MDA in the sample or standard competes with a fixed amount of biotin-labeled MDA for sites on a pre-coated antibody specific to MDA. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of MDA in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

Alias: MDA ELISA Kit, Malondialdehyde ELISA Kit
Species: Rat
Range: 7.813-500ng/ml
Sensitivity: 4.688ng/ml
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Competitive ELISA, Coated with Antigen
Detection Wavelength: OD450
Reaction Duration: 2 hours
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with MDA , no obvious cross reaction with other analogues.