Human IL-4 (Interleukin 4) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alias: Interleukin-4 ELISA Kit, IL-4 ELISA Kit, B-cell stimulatory factor 1 ELISA Kit, BSF-1 ELISA Kit, Binetrakin ELISA Kit, Lymphocyte stimulatory factor 1 ELISA Kit, Pitrakinra ELISA Kit, IL4 ELISA Kit
Species: Human
Range: 31.25-2000pg/ml (1pg=29mIU)
Sensitivity: 18.75pg/ml
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Storage: 2-8°C(Sealed), Don't cryopreserve
Specificity: Specifically binds with IL-4 , no obvious cross reaction with other analogues