Human IL31RA (Interleukin-31 receptor subunit alpha) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-31RA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-31RA bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-31RA in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alias: Interleukin-31 receptor subunit alpha ELISA Kit, IL-31 receptor subunit alpha ELISA Kit, IL-31R subunit alpha ELISA Kit, IL-31R-alpha ELISA Kit, IL-31RA ELISA Kit, Cytokine receptor-like 3 ELISA Kit, GLM-R ELISA Kit, hGLM-R ELISA Kit, Gp130-like monocyte receptor ELISA Kit, Gp130-like receptor ELISA Kit, ZcytoR17 ELISA Kit, IL31RA ELISA Kit, CRL3 ELISA Kit, GPL ELISA Kit
Species: Human
Range: 15.625-1000pg/ml
Sensitivity: 9.375pg/ml
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with IL-31RA , no obvious cross reaction with other analogues.