Human AFP (alpha Fetoprotein) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AFP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with AFP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of AFP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alias: Alpha-fetoprotein ELISA Kit, Alpha-1-fetoprotein ELISA Kit, Alpha-fetoglobulin ELISA Kit, AFP ELISA Kit, HPAFP ELISA Kit
Species: Human
Range: 0.313-20ng/ml
Sensitivity: 0.188ng/ml
Sample Type: Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with AFP , no obvious cross reaction with other analogues.