Human Gp130/IL6ST (Interleukin-6 receptor subunit beta) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Gp130/IL6ST antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Gp130/IL6ST bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Gp130/IL6ST in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alias: Interleukin-6 receptor subunit beta ELISA Kit, IL-6 receptor subunit beta ELISA Kit, IL-6R subunit beta ELISA Kit, IL-6R-beta ELISA Kit, IL-6RB ELISA Kit, CDw130 ELISA Kit, Interleukin-6 signal transducer ELISA Kit, Membrane glycoprotein 130 ELISA Kit, gp130 ELISA Kit, Oncostatin-M receptor subunit alpha ELISA Kit, IL6ST ELISA Kit
Species: Human
Range: 0.25-16ng/ml
Sensitivity: 0.15ng/ml
Sample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Sandwich ELISA, Double Antibody
Detection Wavelength: OD450
Reaction Duration: 4 hours
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with Gp130/IL6ST , no obvious cross reaction with other analogues.