FLAG-Tag (DYKDDDDK-Tag Protein) ELISA Kit

This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with FLAG-Tag. FLAG-Tag in the sample or standard competes with a fixed amount of biotin-labeled FLAG-Tag for sites on a pre-coated antibody specific to FLAG-Tag. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of FLAG-Tag in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

Alias: FLAG-Tag ELISA Kit, DYKDDDDK-Tag Protein ELISA Kit
Species: Universal
Range: 15.625-1000ng/ml
Sensitivity: 9.375ng/ml
Sample Type: Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method: Competitive ELISA, Coated with Antigen
Detection Wavelength: OD450
Reaction Duration: 2 hours
Storage: 2-8°C(Sealed), Don't cryopreserve.
Specificity: Specifically binds with FLAG-Tag , no obvious cross reaction with other analogues.