NEXT 12 Prestained Protein Ladder (12 bands, 5 colors)

The NEXT 12 Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 250 kDa. The 12 recombinant proteins are covalently coupled with a blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa, and a purple band at 250 kDa serve as reference bands. The NEXT 12 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Storage Buffer :
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control :
5 μl of NEXT 12 Prestained Protein Ladder (PMX12) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.