{"product_id":"ae43","title":"Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit","description":"\u003cp\u003eThis kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF-α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNF-α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNF-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e\n\u003cp\u003eAlias: Tumor necrosis factor ELISA Kit, Cachectin ELISA Kit, TNF-alpha ELISA Kit, Tumor necrosis factor ligand superfamily member 2 ELISA Kit, TNF-a ELISA Kit, Tumor necrosis factor, membrane form ELISA Kit, N-terminal fragment ELISA Kit, NTF ELISA Kit, Intracellular domain 1 ELISA Kit, ICD1 ELISA Kit, Intracellular domain 2 ELISA Kit, ICD2 ELISA Kit, C-domain 1 ELISA Kit, C-domain 2 ELISA Kit, Tumor necrosis factor, soluble form ELISA Kit, TNF ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit\u003cbr\u003eSpecies: Mouse\u003cbr\u003eRange: 3.906-250pg\/ml (1pg=270mIU)\u003cbr\u003eSensitivity: 2.344pg\/ml\u003cbr\u003eSample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples\u003cbr\u003eDetection Method: Sandwich ELISA, Double Antibody\u003cbr\u003eDetection Wavelength: OD450\u003cbr\u003eReaction Duration: 4 hours\u003cbr\u003eStorage: 2-8°C(Sealed), Don't cryopreserve\u003cbr\u003eSpecificity: Specifically binds with TNF-α , no obvious cross reaction with other analogues\u003c\/p\u003e","brand":"Finetest","offers":[{"title":"96T","offer_id":47776856473818,"sku":"EM0183-96T","price":608.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/EM0183-1.webp?v=1775808345","url":"https:\/\/q2ub.com\/products\/ae43","provider":"Q2UB","version":"1.0","type":"link"}