{"product_id":"ae25","title":"Human IL-4 (Interleukin 4) ELISA Kit","description":"\u003cp\u003eThis kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e\n\u003cp\u003eAlias: Interleukin-4 ELISA Kit, IL-4 ELISA Kit, B-cell stimulatory factor 1 ELISA Kit, BSF-1 ELISA Kit, Binetrakin ELISA Kit, Lymphocyte stimulatory factor 1 ELISA Kit, Pitrakinra ELISA Kit, IL4 ELISA Kit\u003cbr\u003eSpecies: Human\u003cbr\u003eRange: 31.25-2000pg\/ml (1pg=29mIU)\u003cbr\u003eSensitivity: 18.75pg\/ml\u003cbr\u003eSample Type: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples\u003cbr\u003eDetection Method: Sandwich ELISA, Double Antibody\u003cbr\u003eDetection Wavelength: OD450\u003cbr\u003eReaction Duration: 4 hours\u003cbr\u003eStorage: 2-8°C(Sealed), Don't cryopreserve\u003cbr\u003eSpecificity: Specifically binds with IL-4 , no obvious cross reaction with other analogues\u003c\/p\u003e","brand":"Finetest","offers":[{"title":"96T","offer_id":47776855851226,"sku":"EH0199-96T","price":608.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/EH0199-1.webp?v=1775786657","url":"https:\/\/q2ub.com\/products\/ae25","provider":"Q2UB","version":"1.0","type":"link"}