{"product_id":"ae12","title":"FLAG-Tag (DYKDDDDK-Tag Protein) ELISA Kit","description":"\u003cp\u003eThis kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with FLAG-Tag. FLAG-Tag in the sample or standard competes with a fixed amount of biotin-labeled FLAG-Tag for sites on a pre-coated antibody specific to FLAG-Tag. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of FLAG-Tag in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e\n\u003cp\u003eAlias: FLAG-Tag ELISA Kit, DYKDDDDK-Tag Protein ELISA Kit\u003cbr\u003eSpecies: Universal\u003cbr\u003eRange: 15.625-1000ng\/ml\u003cbr\u003eSensitivity: 9.375ng\/ml\u003cbr\u003eSample Type: Cell Culture Supernatant, cell or tissue lysate, Other liquid samples\u003cbr\u003eDetection Method: Competitive ELISA, Coated with Antigen\u003cbr\u003eDetection Wavelength: OD450\u003cbr\u003eReaction Duration: 2 hours\u003cbr\u003eStorage: 2-8°C(Sealed), Don't cryopreserve.\u003cbr\u003eSpecificity: Specifically binds with FLAG-Tag , no obvious cross reaction with other analogues.\u003c\/p\u003e","brand":"Finetest","offers":[{"title":"96T","offer_id":47776855425242,"sku":"EU2607-96T","price":667.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/EU2607-1.webp?v=1775723448","url":"https:\/\/q2ub.com\/products\/ae12","provider":"Q2UB","version":"1.0","type":"link"}