{"title":"Protein Biology","description":"\u003cp\u003eCategory\u003c\/p\u003e","products":[{"product_id":"s2","title":"MinuteTM Protein Extraction Kit for Hair and Nails","description":"\u003cp\u003eProteins account for about 80% of keratinized animal tissues such as hair, nail, horn and wool. These tissues contain mainly hard keratins made of several distinct types of protein with a molecular weight ranging from 10 to 135 kDa. The extraction buffer usually contains a high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective, but because of larger extraction buffer\/tissue ratio, the concentration of extracted protein is usually low. Extracted proteins need to be concentrated prior to further analysis. The presence of high-concentration denaturants may also interfere with downstream application. HD-021 is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants. The extracted protein can be directly loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other chemicals. Extracted protein concentration is 1-2 mg\/ml.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116175085786,"sku":"HD-021","price":885.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/HD-021_17b4d4b8-df2a-4f9c-8ea4-9c15b8d9dc75.webp?v=1758812010"},{"product_id":"s3","title":"MinuteTM Total Protein Extraction Kit for Mass Spectrometry","description":"\u003cp\u003eMass spectrometry has become a popular method for analyzing and characterizing proteins. Traditional total protein extraction methods, such as RIPA-based reagents, can alter endogenous baselines due to nonsystematic protein loss. Some chemicals in RIPA may not be compatible with mass spectrometry and other downstream applications. This kit extracts total proteins quickly using a specially formulated lysis buffer and proprietary spin column. The extracted proteins are compatible with trypsin digestion followed by MS analysis, iTRAQ, biotin labeling, etc. Due to the use of filter cartridges, the extraction volume can be as low as 20 µl – a helpful feature when starting material is a limiting factor. The typical yield is 2-8 mg\/ml.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175315162,"sku":"MS-026","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/MS-026_242e670b-b524-48d2-8f9d-c8f30c4e89f6.webp?v=1758812012"},{"product_id":"s4","title":"MinuteTM Total Protein Extraction Kit for Skin Tissue","description":"\u003cp\u003eSkin tissue is made up of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal skin tissues by using a combination of mechanical extraction and chemical lysis of skin tissues. The kit features a simple and fast single-tube protocol and optimized buffers for skin tissues. The researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for skin tissue. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-5 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175347930,"sku":"SA-01-SK","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-01-SK_6eeb38e7-08fb-4407-ad02-0b9e391294c4.webp?v=1758812015"},{"product_id":"s5","title":"MinuteTM Total Protein Extraction Kit for Bone Tissue","description":"\u003cp\u003eBone tissues are commonly used for research. There are two types of bone tissues: compact and spongy. Cells in bone tissues are tightly packed and, because of their unique structures, it is very difficult to efficiently extract total protein from them. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for extracting proteins from human or animal bone tissue by a combination of mechanical extraction and chemical lysis of cells in tissues. The kit features a simple and fast single-tube protocol and optimized buffers for bone tissue. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for bone tissue. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 0.5-2 mg\/ml depending upon type of bones. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175380698,"sku":"SA-02-BT","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-02-BT_98728523-83a0-4906-96e0-aab07ac753a6.webp?v=1758812017"},{"product_id":"s6","title":"MinuteTM Total Protein Extraction Kit for Blood Vessels","description":"\u003cp\u003eBlood vessels (arteries and veins) are made of specialized connective tissues. There are three layers of tissues in blood vessels: tunica, tunica media and endothelium. Due to their unique structures, blood vessels are notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased with solution-based (incomplete protein profile) methods. This kit provides a highly efficient method for total protein extraction from human or animal blood vessels by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than the RIPA buffer. The kit features a simple, fast single-tube protocol and optimized buffers for blood vessels. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for blood vessels. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175413466,"sku":"SA-03-BV","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-03-BV_b97e0b87-6cfe-46dd-8d93-8fea4e705790.webp?v=1758812020"},{"product_id":"s7","title":"MinuteTM Total Protein Extraction Kit for Tendons","description":"\u003cp\u003eA tendon\/ligament is a fibrous connective tissue that attaches muscles to bone. Histologically tendons consist of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also very difficult to lyse the tendon tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein is also biased (incomplete protein profile) with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal tendons by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for tendons. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for tendons. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175446234,"sku":"SA-04-TD","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-04-TD_71aafbcc-fe9e-4e1b-ac34-f5a699e26a65.webp?v=1758812023"},{"product_id":"s8","title":"MinuteTM Total Protein Extraction Kit for Intestines and Mesentery","description":"\u003cp\u003eThe intestines are a long and continuous tube that run from stomach to anus. Mesentery is a fold of tissue that attaches intestines to the body wall. Structurally, intestines and mesentery are very different. However, from a protein extraction point of view, they have one thing in common: they are very tough and hard to homogenize. It is also very difficult to lyse cells in the tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from human or animal intestines and mesentery tissues using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for intestine and mesentery tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer for specific downstream applications. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175479002,"sku":"SA-05-IM","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-05-IM_cb09db88-e61c-4411-accb-87c232f65ebf.webp?v=1758812025"},{"product_id":"s9","title":"MinuteTM Total Protein Extraction Kit for Muscles","description":"\u003cp\u003eSkeletal and cardiac muscles are highly specialized tissues that are made of well-arranged muscle fibers.  An individual muscle may be made up of hundreds or even thousands of muscle fibers bundled together and wrapped in a connective tissue covering. The unique organization of the muscle tissue makes it very hard to homogenize. It is also very difficult to lyse cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from human\/animal skeletal or cardiac muscle tissues using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for muscle tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for muscle tissues. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 2-10 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175511770,"sku":"SA-06-MS","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-06-MS_ae99322f-8d1a-4912-bfa5-30fc05cdf185.webp?v=1758812028"},{"product_id":"s10","title":"MinuteTM Total Protein Extraction Kit for Insects","description":"\u003cp\u003eDespite significant variation in body organization, insects all have the same general body structure. They have segmented bodies divided into three regions: head, thorax and abdomen. The body segments are protected by a hard exoskeleton or cuticles. From a protein extraction point of view, the unique structure of an exoskeleton makes it very hard to homogenize. It is also very difficult to lyse cells protected by cuticle for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from insects using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for insect tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for insects. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg\/ml. The materials provided are sufficient for 50 extractions.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175544538,"sku":"SA-07-IS","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SA-07-IS_f95cb5ea-30f2-489c-8df1-bab7595ee726.webp?v=1758812031"},{"product_id":"s11","title":"Minute TM Total Protein Extraction Kit  for Animal Cultured Cells\/Tissues","description":"\u003cp\u003eMinuteTM total protein extraction kit for animal cultured cells and tissues is the most advanced next-generation tool for super-fast protein extraction without altered protein profile usually associated with solution-based procedures. More and more evidence has shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved using the patented spin column-based technologies. Coupled with stronger lysis buffers, the spin column makes protein extraction super easy and efficient. The extraction volume can be as low as 20 µl – a helpful feature when starting material is a limiting factor. This kit provides denaturing and native cell lysis buffers so users can select according to specific applications. Total proteins can be extracted from cultured cells\/tissues in 1-8 min with high yield (2-8 mg\/ml).\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175642842,"sku":"SD-001\/SN-002","price":885.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SD-001_SN-002_03d96c9a-8e9c-4900-b819-92219358555e.webp?v=1758812034"},{"product_id":"s12","title":"Minute TM Total Protein Extraction Kit for Plant Tissues","description":"\u003cp\u003eThe MinuteTM total protein extraction kit for plant tissues comprises an optimized protein extraction buffer and protein extraction filter cartridges accompanied by 2.0 ml collection tubes. It aims to efficiently extract denatured or native proteins from various plant tissues, including leaves, seeds, soft stems, and roots. Notably, the protein profiles obtained through denaturing and native cell lysis buffers differ. Therefore, depending on the specific application, one buffer may outperform the other. This kit offers both denaturing and native cell lysis buffers, allowing users to evaluate and select the most suitable option for their particular needs. Utilizing the protein extraction filter cartridges makes it possible to extract total plant soluble proteins from 50-200 mg of plant tissue within 5-8 minutes while achieving a high protein yield ranging from 2-8 mg\/ml. This extraction method is useful for SDS-PAGE, immunoblotting, ELISA, IP, and enzyme assays. These extracted proteins can also be excellent starting materials for small-scale protein purification using column chromatography techniques.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175675610,"sku":"SD-008\/SN-009","price":885.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SD-008_SN-009_e7c2b4ff-0cf0-49dc-b966-9ccc78937ca7.webp?v=1758812036"},{"product_id":"s13","title":"MinuteTM Thylakoid Enrichment and Chloroplast Fractionation Kit","description":"\u003cp\u003eA chloroplast, delimited by a two-membrane envelope, is a type of plastid that serves as the site of photosynthesis. The photosynthetic function is based on developing an operational extensive internal membrane network, called the thylakoids, and on enzymatic processes in the chloroplast matrix, called the stroma. Thylakoid membrane is structurally different from the chloroplast envelope, and their biogenesis depends on biosynthetic and transporting activities specific to the chloroplast envelope. This kit is designed to fractionate chloroplasts into three parts (thylakoid membrane, envelope membrane, and stroma) using a specialized filter cartridge and differential centrifugation (without ultracentrifugation). Isolated thylakoids are in native forms and can be used for functional studies and the studies of subcellular localization of proteins using Western blotting\/Mass spectrometry. The buffers in the kit are detergent and EDTA-free. The protocol can be completed in about one hour.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 preps","offer_id":46116175708378,"sku":"CF-055","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/CF-055_d56c799d-bea5-4fc1-9d97-1cd9e73be639.webp?v=1758812040"},{"product_id":"s14","title":"Minute TM Chloroplast Isolation Kit","description":"\u003cp\u003eInvent Biotechnologies MinuteTM chloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 50-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 106 to 1 X 107 intact chloroplasts (\u0026gt;90% intact) from fresh plant leaves.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116175741146,"sku":"CP-011","price":901.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/CP-011_6b275363-799d-4f1d-b49b-745c7c204325.webp?v=1758812042"},{"product_id":"s15","title":"MinuteTM Endosome Isolation and Cell Fractionation Kit","description":"\u003cp\u003eEarly endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and is tedious and time-consuming. The Minute™ Endosome Isolation and Cell Fractionation Kit provides a spin-column-based novel endosome isolation technology that is rapid and simple and requires a smaller number of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116175773914,"sku":"ED-028","price":1441.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/ED-028_8a126624-7d1d-4827-ba7f-3b23358b6b45.webp?v=1758812045"},{"product_id":"s16","title":"MinuteTM Hi-Efficiency Exosome Isolation Reagent (Non-PEG)","description":"\u003cp\u003eExosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm. The biological function of exosomes is believed to serve as intercellular messengers. Filtration and ultracentrifugation are classical ways of isolating and enriching exosomes. This is a tedious process and requires special equipment. Another common way for enrichment of exosome is through precipitation. Currently major commercial kits are PEG-based. EI-027 is designed to precipitate total exosomes from biofluids using a high efficacy, non-PEG based reagent for exosome precipitation. This kit is suitable for routine biofluid samples using the same reagent and similar protocol.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 ml","offer_id":46116175970522,"sku":"EI-027","price":901.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/EI-027_de903c20-f2ea-4831-bf80-a8e7a3919494.webp?v=1758812047"},{"product_id":"s17","title":"MinuteTM ER Enrichment Kit","description":"\u003cp\u003eThe endoplasmic reticulum (ER) is a significant membranous structure that bridges the nuclear membrane and the plasma membrane, serving as a pivotal player in the exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in the cytoplasm are directed to the ER, from where vesicles transport protein cargo to the Golgi apparatus, leading to subsequent fusion with the plasma membrane.\u003c\/p\u003e\n\n\u003cp\u003eTraditional ER isolation methods rely on density gradient ultracentrifugation, a process demanding a substantial amount of starting material. These methods can be laborious, time-consuming, and often result in notable cross-contamination. Remarkably, all existing commercial kits for ER isolation are based on techniques developed in the 1970s. In a departure from these traditional approaches, the Minute™ ER enrichment kit stands out in the market by harnessing patented spin-column-based technology. This innovative method is not only straightforward and rapid but also requires only a small quantity of starting cultured cells or tissues.\u003c\/p\u003e\n\n\u003cp\u003eThis kit excels at differentially precipitating native ER, primarily rough ER, from cultured cells and tissues, all without the need for a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in approximately two hours.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116176101594,"sku":"ER-036","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/ER-036_77901ac0-cd50-4c80-a99c-3da7803048da.webp?v=1758812051"},{"product_id":"s18","title":"MinuteTM Golgi Apparatus Enrichment Kit","description":"\u003cp\u003eThe Golgi apparatus, also known as the Golgi complex or Golgi body, consists of a series of flattened stacked pouches called cisternae. This organelle plays a crucial role in eukaryotic cells by facilitating the transportation, modification, and packaging of proteins and lipids into vesicles for delivery to specific locations. The quantity and distribution of Golgi vary significantly across different cell and tissue types. Obtaining a highly enriched Golgi fraction is a crucial initial step in the study of its function and interactions with other organelles.\u003c\/p\u003e\n\n\u003cp\u003eTraditional methods for isolating the Golgi apparatus rely on density gradient ultracentrifugation, which demands a substantial amount of starting material and can be laborious and time-consuming. In contrast, the Minute™ kit distinguishes itself from other Golgi isolation kits by employing patented spin-column-based technology. This approach is both straightforward and rapid, requiring only a small amount of starting material. With this kit, native Golgi can be preferentially enriched through precipitation, eliminating the need for a Dounce homogenizer and ultracentrifugation. It enables the isolation of two sub-Golgi fractions: the Golgi apparatus and secretory vesicles of the Golgi.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116176330970,"sku":"GO-037","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/GO-037_c15600b0-0f98-4362-a2a1-3d8bf418504a.webp?v=1758812055"},{"product_id":"s19","title":"Minute™ Total Lipid Raft Isolation Kit for Mammalian Cells\/Tissues","description":"\u003cp\u003eLipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids.  Lipid rafts are found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. They have been implicated in numerous cellular processes including but not limited to signal transduction, membrane trafficking, and protein sorting. Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+\/K+ ATPase on the plasma membrane. Lipid rafts are isolated by sucrose-gradient or OptiPrep gradient traditionally using ultracentrifugation that requires a large amount of starting material. The protocol is tedious and time-consuming. This kit was developed using our proprietary spin-column-based technologies, offering a rapid and easy way to isolate lipid rafts. Total membrane fraction (include PM and organelle membranes) is first isolated and treated with a non-ionic detergent containing buffer, followed by isolation of detergent-resistant fraction by flotation centrifugation using just a tabletop microcentrifuge. Highly enriched total lipid rafts can be obtained from cultured cells\/tissues in less than 90 min without using density gradient and ultracentrifugation.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116176396506,"sku":"LR-039","price":1340.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/LR-039_44cdef33-d7b5-451c-8808-14446df869a4.webp?v=1758812057"},{"product_id":"s20","title":"Minute™ Plasma Membrane-Derived Lipid Raft Isolation Kit","description":"\u003cp\u003eLipid rafts are small membrane domains containing a high level of cholesterol and sphingolipids. Lipid rafts have been found in the plasma membrane (PM) and internal organellar membranes such as mitochondria-associated membrane (MAMs) and endoplasmic reticulum. Lipid rafts are implicated in numerous cellular processes such as signal transduction, membrane trafficking, and protein sorting.  Lipid-modified proteins and some transmembrane proteins are concentrated in the rafts while other proteins are excluded. Lipid rafts are also found to be associated with Na+\/K+ ATPase on PM. Traditional methods for lipid raft isolation involve isolation of detergent-resistant membrane subdomain from total membranous structures, which does not distinguish plasma membrane-derived and\/or organelle-derived lipid rafts. Using the patented spin-column-based technologies, we have developed this kit specifically for the isolation of plasma membrane-derived lipid rafts. Larger plasma membrane vesicles are first isolated and treated with a non-ionic detergent containing buffer followed by isolation of detergent-resistant fraction by flotation centrifugation using a tabletop microcentrifuge. Highly enriched plasma membrane-derived lipid rafts can be obtained in about 1 hour without using a traditional homogenizer and ultracentrifugation.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116176593114,"sku":"LR-042","price":1340.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/LR-042_43fe25fa-7a48-4218-8e42-91552a3ff945.webp?v=1758812061"},{"product_id":"s21","title":"MinuteTM Lysosome Isolation Kit","description":"\u003cp\u003eLysosomes, found in eukaryotic cells, are spherical vesicles responsible for the removal of cellular waste. These organelles contain digestive enzymes crucial for breaking down excess or worn-out organelles, food particles, as well as viruses and bacteria that have been engulfed. Lysosomes exhibit a relatively large size, ranging from 0.1 to 1.2 micrometers. In the realm of cell research, the initial isolation of lysosomes is a pivotal step when investigating processes like autophagy, protein degradation, and recycling.\u003c\/p\u003e\n\n\u003cp\u003eTraditional methods for lysosome isolation, which originated in the 1970s, are associated with challenges such as the need for substantial starting material and a time-consuming procedure that often leads to significant cross-contaminations. However, a groundbreaking solution is offered by this kit, utilizing patented spin-column-based technology that is characterized by its simplicity, speed, and efficiency. With this kit, native lysosomes can be notably enriched without the requirement of a Dounce homogenizer or ultracentrifugation. The entire protocol can be completed in less than 90 minutes, utilizing only 20-30 milligrams of the starting material.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116176658650,"sku":"LY-034","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/LY-034_d67c4b30-b809-4c76-8e06-a2ce89b983cb.webp?v=1758812063"},{"product_id":"s22","title":"Minute™ Plant Microsomal Membrane Extraction Kit","description":"\u003cp\u003eIsolation of microsomal membranes from plant tissues is a common procedure. The microsomal fraction of plant cell lysate is the focus of interest in many plant research projects and is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes, and other components of a membrane system. Traditional methods for microsomal fraction isolation involve differential pelleting, where centrifugation steps are required for membrane fractions. Microsomal fraction isolation usually requires a large amount of starting material and has tedious ultracentrifugation steps. MM-018 offers a simple, fast, and user-friendly approach for microsomal membrane extraction with a small amount of starting material. During the procedures, water-soluble cytosolic proteins are removed, and water-insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a tabletop microcentrifuge. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg\/sample.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116176756954,"sku":"MM-018","price":1391.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/MM-018_adb99be3-9327-4f62-8bc4-a138fa4c60bb.webp?v=1758812066"},{"product_id":"s23","title":"Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues","description":"\u003cp\u003eMinute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues comprises optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to protein extraction filter cartridges, the protein isolation procedure is simple and provides a high yield. The process can be completed in less than 30 minutes. Unlike many commercial mitochondria preparation kits, this kit offers a wide range of starting cells and isolated mitochondria. The buffers are detergent and EDTA-free. A Dounce homogenizer or a tissue blender is not needed.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116176855258,"sku":"MP-007","price":1256.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/MP-007_8e49334f-c6b1-44a3-ab3a-c90021f49a33.webp?v=1758812069"},{"product_id":"s24","title":"Minute TM Nuclear Envelope Protein Extraction Kit","description":"\u003cp\u003eThe nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure. Minute™ Nuclear Envelope Protein Extraction Kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultracentrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods that require large amounts of starting cells\/tissues, this kit starts with only 10-20 million cells, and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 minutes with a final yield of 10-50 µg protein\/sample.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116177805530,"sku":"NE-013","price":1509.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/NE-013_56631b02-c2eb-4931-bc1c-cba90e18e95a.webp?v=1758812072"},{"product_id":"s25","title":"MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues","description":"\u003cp\u003ePreparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. The majority of current commercial kits are designed to treat cultured cells and tissues similarly, not paying sufficient attention to the structural difference between the two. As a result, cross-contamination of cytosolic and nuclear fractions from fresh\/frozen tissues remains a major problem (see references 1 and 2 below). This novel Cytosolic and Nuclear Extraction Kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contamination for fresh and frozen tissue samples.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116177838298,"sku":"NT-032","price":1028.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/NT-032_1827be24-8b76-4bf4-8087-6cdd58f01894.webp?v=1758812075"},{"product_id":"s26","title":"MinuteTM Plant Endosome Enrichment Kit","description":"\u003cp\u003eAn understanding of endomembrane processes and their biological implication is important for understanding plant growth and development. Endosomes are a heterogeneous collection of organelles in the sorting and delivery of internalized material from cell surface and the transport of materials from Golgi to lysosome or vacuole. In plant cells, two clearly defined endosomal compartments have been identified: the trans-Golgi network (early endosome equivalent) and the multivesicular body (late endosome equivalent). Traditionally, methods for isolation and purification of these two compartments are tedious and time-consuming involving sucrose gradient centrifugation. In some cases, sucrose gradient isolation is coupled with immunoaffinity purification and larger amount of starting materials are usually required (10-50 g). We have developed this kit using a spin-column-based format coupling with selective precipitation for enrichment of endosomal compartment. This approach is easy and rapid and only small amount of starting material is required.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116177969370,"sku":"PE-050","price":941.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PE-050_5b337e91-53ff-47f5-b23c-b848ab5240ab.webp?v=1758812078"},{"product_id":"s27","title":"MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit","description":"\u003cp\u003eThe kit is designed for rapid fractionation of fresh\/frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100-200 mg starting material.  By using a specialized filter cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol, nuclei, chloroplasts and organelles) can be obtained in about 1 hour. The subcellular fractions can be used in numerous applications that include but not limited to protein trafficking, nuclear protein extraction and nucleic acid purification\/sequencing. The highly enriched nuclei can be used for proteomics, flow cytometry analysis, DNA sequencing, and protein-protein\/protein-DNA interaction studies. The subcellular fractionation protocol includes grinding tissue to release plant cells followed by disruption of the cell membrane by rapidly passing through the filter. Different subcellular fraction is obtained by differential centrifugation using only a table top centrifuge. This kit has been tested for use with many species such as Arabidopsis, Spinach, Peas, and Rape.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116178002138,"sku":"PF-045","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PF-045_7f65fb0a-1c4e-4db2-b647-f15f0a0069d4.jpg?v=1758812082"},{"product_id":"s28","title":"MinuteTM Plant Golgi Apparatus Enrichment Kit","description":"\u003cp\u003eThe plant Golgi apparatus plays an important role in the biosynthesis of cellular structural components and protein trafficking. However proteomic characterization of the Golgi has been hindered by limited methodologies for obtaining enriched\/purified Golgi fraction. The traditional method for isolating Golgi apparatus depends upon the use of density centrifugation, which is tedious and time-consuming. A large amount of starting material is usually required. Plant Golgi stacks are notoriously labile and loss of stack integrity can occur if harsh homogenization is used. This means that assessment of purity by morphological means such as electron microscopy is difficult. This kit is designed to overcome certain disadvantages of traditional methods by employing spin column-based homogenization, differential centrifugation, and selective precipitation for the enrichment of the Golgi. The protocol is simple and straightforward and requires only a milligram amount of starting material. Significant enrichment of the Golgi fraction (2-3 folds) can be obtained.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116178952410,"sku":"PG-049","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PG-049_0c2dc359-3149-4d92-8373-aa0b4c61aefb.webp?v=1758812085"},{"product_id":"s29","title":"MinuteTM Plant Lipid Raft Isolation Kit","description":"\u003cp\u003eThere are substantial evidences that support the existence of detergent-resistant membrane (DRM) subdomains in both animal and plant membrane systems, which is also referred to as lipid rafts. Lipid rafts are shown to have increased sphingolipid to protein ratio and higher cholesterol concentration. The lipid rafts are believed to play an important role in signal transduction and protein trafficking in eukaryotic cells. Traditional method for lipid raft isolation involves cold non-ionic detergent extraction followed by sucrose gradient ultracentrifugation. The protocol requires large amount of starting material (hundred grams amount) and specialized equipment in addition to lengthy protocol time. We have developed a rapid method for isolation of lipid rafts from plant tissues using only milligram amount of starting material and the protocol can be completed in about 1h without using ultracentrifugation.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116179017946,"sku":"PL-051","price":1054.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PL-051_25736bd3-5c34-4af3-87b1-2141ca55a9f4.webp?v=1758812088"},{"product_id":"s30","title":"MinuteTM Nuclear Proteasome Enrichment Kit","description":"\u003cp\u003eThe proteasome is a protein degradation system that requires metabolic energy and polymerization of ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). The proteasomes have been found mainly localized in the cytosol but also detected in the nucleus. Traditionally, ultracentrifugation and affinity isolation are the most common methods for the isolation of proteasomes. These methods, though relatively effective, are usually time-consuming with low yield. Many affinity-based methods require harsh elution conditions that may affect the activity of isolated proteasomes and limit certain downstream applications. In most cases, the methods can’t distinguish proteasomes derived from cytosol or nucleus. To overcome these shortcomings, we have developed spin-column-based kits for the enrichment of proteasome from nuclei (Cytosolic Proteasome Enrichment Kit is also available under Cat # PT-040). The protocol is simple and rapid with a high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins and is useful for the studies of proteasome structure and function. The highly enriched proteasomes can also be used as a starting material for affinity purifications.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116179116250,"sku":"PN-041","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PN-041_58f2f735-12df-4569-84b5-ffcfb41987bb.jpg?v=1758812092"},{"product_id":"s31","title":"MinuteTM Plant ER Enrichment Kit","description":"\u003cp\u003eIsolation and enrichment of plant endoplasmic reticulum (ER) are essential for elucidating ER-related cellular functions. The traditional method for ER isolation is tedious and time-consuming.  In animal cells, ER is closely associated with microtubules, and Golgi apparatus are clustered at microtubule organizing center. The ER is associated with actin microfilaments in plant cells, and no microtubule organizing center is found near the nuclei. Due to the structural characteristics of plan ER, it is much more challenging to isolate\/enrich plant ER than animal samples. Minute™ plant ER enrichment kit was specially designed for plants. One to three-fold enrichment of ER fraction can be obtained from plant leave samples in about 1 hour. The enriched ER is essentially free from Golgi apparatus contamination. The enriched ER fraction can be used in plant protein trafficking studies.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116179181786,"sku":"PR-048","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PR-048_2c8bf332-4e8f-4db0-8259-2730a98226be.webp?v=1758812094"},{"product_id":"s32","title":"MinuteTM Cytosolic Proteasome Enrichment Kit","description":"\u003cp\u003eThe proteasome is a non-lysosome protein degradation system that requires metabolic energy and polymerization of ubiquitin. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). Rapid and gentle isolation of proteasome from cells\/tissues is essential for the studies of proteasome structure and function. The proteasomes have been found mainly localized in cytosol but also detected in nucleus. Traditionally, ultracentrifugation and affinity isolation are the most common methods for isolation of proteasomes. These methods, though relatively effective, are usually time consuming with low yield. Many affinity-based methods require harsh elution condition that may affect the activity of isolated proteasomes and limit certain downstream applications. To overcome these shortcomings, we have developed a spin-column based technology using a proprietary precipitation buffer for enrichment of proteasome from cytosol by first removing nuclei and majority of organelles followed by preferential precipitation of cytosolic proteasomes. The nuclear proteasomes are excluded with this kit (Nuclear Proteasome Enrichment Kit is available under Cat # PN-041). The protocol is simple and rapid with high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins. This kit is useful for the studies of proteasome structure and function. The enriched proteasomes can also be used as a starting material for affinity purification.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116179280090,"sku":"PT-040","price":1171.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PT-040_44ad9044-28b9-42be-917e-8d209832416e.webp?v=1758812098"},{"product_id":"s33","title":"MinuteTM  Plasma Membrane Isolation Kit for Mammalian Red Blood Cells NEW!","description":"\u003cp\u003eRed blood cells (RBCs) are essential components of the circulatory system, responsible for transporting oxygen and carbon dioxide throughout the body. Understanding their surface composition, particularly the diverse array of proteins is crucial for research in blood biology and disease. This protocol offers a rapid and efficient method for isolating native plasma membranes (PM) vesicles from RBCs with associated membrane proteins. The method utilizes differential centrifugation and a specialized protein extraction powder for gentle RBC lysis while enriching for PM fractions. Importantly, this approach preserves the native protein conformation and avoids the use of detergents, facilitating for downstream studies of membrane-associated proteins. The entire protocol can be completed in approximately 1 hour, yielding highly enriched PM with minimal contamination from intracellular components like spectrin and hemoglobin.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116179312858,"sku":"RP-057","price":1094.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/RP-057_a60b6309-dcfc-4f27-9e91-f63544b23671.webp?v=1758812101"},{"product_id":"s34","title":"Minute TM Cytoplasmic and Nuclear Fractionation kit","description":"\u003cp\u003eMinute™ Cytoplasmic and Nuclear Extraction Kit for Cells comprises optimized cytoplasmic extraction buffer, nuclear protein extraction buffer, and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plants, bacteria, yeast, and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116179378394,"sku":"SC-003","price":1028.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SC-003_80f1dfda-5122-4643-8be7-1929f69f2096.webp?v=1758812105"},{"product_id":"s35","title":"MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (Non-PEG)","description":"\u003cp\u003eDiagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris, large amount of amylase, mucin and glycoprotein are present in saliva, and make the samples viscous and hard to manipulate. Pre-treatments, such as sonication and dilution, are often required. In many cases, the saliva samples can still be difficult to handle even after pre-treatments. This product is specifically designed to address these issues using the proprietary saliva filters. Highly viscous saliva samples can be converted to non-viscous solutions instantly by passing the samples through the filter. Exosomes can then be readily precipitated from as little as 100 µl saliva using the highly effective non-PEG-based reagent.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116179607770,"sku":"SE-030","price":901.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SE-030_8597f02c-8583-42c9-92c2-b823eec6e80e.webp?v=1758812107"},{"product_id":"s36","title":"Minute TM Plasma Membrane Protein Isolation Kit","description":"\u003cp\u003eThe MinuteTM kit represents the next-generation product for plasma membrane (PM) isolation and cell fractionation, offering unsurpassed convenience and consistency by eliminating uncontrollable variations associated with traditional homogenization and density gradient centrifugation, including two-phase partitioning.\u003c\/p\u003e\n\n\u003cp\u003eHow it works: cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied, resulting in a cell lysate containing ruptured cell membranes and intact nuclei. As a result, nuclear contamination is virtually eliminated. PM is further separated from the cell lysate (a mixture of crude membranes, intact nuclei, cytosol proteins, and organelles) by subsequent differential and density centrifugation with a regular tabletop microcentrifuge. 5 distinct cell fractions (total membrane, PM, cytosol, nucleus, and organelles) can be obtained. The procedure can be completed in less than 45 minutes.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116179640538,"sku":"SM-005","price":1279.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SM-005_b98b3e1f-69d9-4b25-b73e-9482be7bd704.webp?v=1758812110"},{"product_id":"s37","title":"Minute TM Plasma Membrane Protein Isolation Kit for Plants","description":"\u003cp\u003ePlasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning. These relatively effective methods require ultracentrifugation and a large amount of starting material. The procedures are usually tedious and time-consuming. To overcome the shortcomings, we have developed this PM isolation kit. Plant tissues are first sensitized by buffer A, homogenized and then passed through a specialized filter cartridge that allows homogenates to pass through with a zigzag path. The cell membranes are ruptured into a range of predefined sizes during the process. Native plasma membranes are separated from a mixture of unruptured cells, nuclei, cytosol and organelles by subsequent differential centrifugation and density centrifugation without using ultracentrifugation. Due to the use of the same amount of starting material, defined centrifugal force and predefined duration in every experiment, the result is much more consistent with a high degree of PM protein enrichment. The procedure can be completed in about 1 hour.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116179673306,"sku":"SM-005-p","price":1279.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SM-005-P_424a34b6-520f-4dfe-bfe6-7583155563a8.webp?v=1758812112"},{"product_id":"s38","title":"MinuteTM Synaptosome Isolation Kit","description":"\u003cp\u003eThe ability to isolate synaptosomes from neuronal tissues\/cultured cells is essential for understanding the mechanisms of neurological diseases. Contained in synaptosomes are synaptic vesicles with diameters of 80-200 nm that play an important role in signal transmission. Traditionally, synaptosomes are isolated by density ultracentrifugation. The procedure is relatively tedious and time-consuming. A larger amount of starting material is usually required. MinuteTM synaptosome isolation kit provides a simple and rapid method for isolating synaptosomes from fresh\/frozen neuronal tissues\/cultured cells. The protocol can be completed in less than 1 h without using Dounce homogenizer and ultracentrifugation. The amount of starting material required (10-50 mg tissue) is only a fraction of that required by the traditional method. The buffers used are detergent-free and synaptosome associated proteins are isolated in native form.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116179837146,"sku":"SY-052","price":885.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SY-052_a9f2e4f1-98e6-47fa-8879-e2fe1a5da498.webp?v=1758812115"},{"product_id":"s39","title":"MinuteTM  Yeast\/Fungus Nuclear and Cytoplasmic Protein Extraction Kit NEW!","description":"\u003cp\u003eThe Minute™ Yeast\/Fungus Nuclear and Cytoplasmic Protein Extraction Kit offers a fast and reliable method to isolate both cytosolic and nuclear proteins from yeast and fungus. This easy-to-use kit allows you to separate these crucial cellular components in about 1h, streamlining your research workflow. The kit provides all the necessary buffers and disposable tools for an efficient protein extraction. Using this kit, you'll have access to both cytosolic and intact nuclei that can be used for nuclear protein and nucleic acid extraction.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180000986,"sku":"YN-058","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/YN-058_1444a9db-8873-4ed8-b212-33ca0d4c928a.webp?v=1758812118"},{"product_id":"s40","title":"MinuteTM Cell Suspension Isolation from Fresh\/Frozen Tissues","description":"\u003cp\u003eObtaining quality cell suspension from fresh and\/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification. Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time consuming. Most importantly, cells in the tissue, especially frozen tissue, are usually damaged and show low integrity and viability. It is very difficult to obtain high viability cell suspension from frozen tissues with high connective tissue contents such as liver, kidney and brain tissues. We have developed a simple, rapid, efficient, and instrument-free method for isolating cell suspension from fresh or frozen tissues using a combination of chemical tissue dissociation and physical separation mechanisms. The protocol can be finished in about 20 min with higher cell integrity and viability.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"50 Preps","offer_id":46116180164826,"sku":"CS-031","price":875.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/CS-031_44ee0151-4a47-43d0-918c-b5d541dccfc7.webp?v=1758812120"},{"product_id":"s41","title":"MinuteTM Nuclei and Cytosol Isolation Kit for Adipose Tissues","description":"\u003cp\u003eAdipocytes are the major energy storage sites in the body, and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into a single nucleus and cytosolic fraction is a common lab practice. However, when it comes to adipose tissue and adipocytes, separating these two fractions is quite challenging due to the low protein content and the presence of abundant lipid droplets. Methods developed by different labs are tedious and unpractical, requiring up to 50 grams of starting materials. This kit provides a quick and simple protocol for obtaining high-quality single nuclei and cytosol fractions from adipose tissue and adipocytes. Only milligrams of starting material are required, making it possible to isolate nuclei and extract proteins from biopsies and limited tissues. The intact nuclei isolated can be used in many applications such as scRNA-seq, ATAC-seq, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, and other applications.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180263130,"sku":"AN-029","price":1272.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/AN-029_ca11cf3f-604e-47d6-85fe-dc5f90a52050.webp?v=1758812124"},{"product_id":"s42","title":"MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues\/Cells","description":"\u003cp\u003eIsolated nuclei are widely used for various experiments such as FACS, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle, and apoptosis research. Single-cell RNA-seq is a powerful technology for studying the complex cellular composition of tissues. However, Neurons are highly interconnected, and it is challenging to obtain single cells from neuronal tissues such as the brain and spinal cord. It is even more difficult to isolate intact cells from frozen neuronal tissues. Due to these limitations, single-cell RNA-seq is being substituted by single-nucleus-seq. The traditional method for single nucleus isolation from neuronal tissue is relatively tedious and time-consuming, and the yield is usually low because it is difficult to get rid of contaminating myelin and other cellular components. This kit is designed to address the issues with a simple, rapid, and effective protocol. The highly purified single nucleus can be obtained in about 30 min. Compared to the traditional method, the kit requires much less starting material and can handle an extensive range of sample sizes (1-25 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not desirable, a detergent-free nuclei isolation kit is also available under cat# NI-024.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180426970,"sku":"BN-020","price":1171.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/BN-020_9d8c117e-fdf1-4d4c-8e7d-bfd3636bbe45.webp?v=1758812127"},{"product_id":"s43","title":"MinuteTM Detergent-Free Nuclei Isolation Kit","description":"\u003cp\u003eThe detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-based technologies in less than 20 min without using tissue homogenizer and any detergents. The traditional method for nuclei isolation involves the use of non-ionic detergent, which has a tendency to cause unwanted nuclear aggregation. It is unclear why some nuclei are aggregated while others are not. It is also unknown whether non-aggregated nuclei are an unbiased representation of the whole nuclei population. Detergents also have the potential to damage the nuclear envelope resulting in leakage of nuclear matrix materials. For some cell\/tissue types, nuclear envelop proteins could also be stripped off by the detergents. MinuteTM Detergent-Free Nuclei Isolation Kit provides a whole new way for nuclei isolation in contrast to traditional methods.\u003c\/p\u003e\n\n\u003cp\u003eHow it works: cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied. The cells are ruptured when passing through the filter leaving intact native nuclei in the flow-through. The nuclei are separated from other small cell debris by low-speed centrifugation using the proprietary buffer B. The native and intact nuclei isolated can be used for a variety of downstream applications that include but not limited to: FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and\/or apoptosis research.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180459738,"sku":"NI-024","price":1104.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/NI-024_9265306e-7b3a-4c8b-8eb7-f0e6643c0000.webp?v=1758812129"},{"product_id":"s44","title":"MinuteTM Single Nuclei Isolation Kit for Plant Tissues","description":"\u003cp\u003eSingle nuclei have become the ideal materials for next-generation genomics and proteomics. However, isolating high-quality single nuclei from plant tissues remains challenging. Most lab-developed methods are limited to specific tissue or species, and no standard protocol and buffer system exist to handle all tissue types from different species. Significant optimization may be needed for different samples. One major drawback of traditional methods is the clumping or aggregation of isolated nuclei, making them difficult for applications such as single nucleus RNA-seq and ATAC analysis. This kit is designed to rapidly isolate well-separated single nuclei from tissues of model and widely-studied plants with minimal clumping using a specially designed spin column and proprietary buffers.  The protocol takes about 30 minutes and requires only 150-200mg of the starting material.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180492506,"sku":"PS-054","price":1104.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/PS-054_9705bb93-27eb-4f08-b12f-bdbea8e30528.webp?v=1758812133"},{"product_id":"s45","title":"MinuteTM Single Nucleus Isolation Kit for Tissues\/Cells","description":"\u003cp\u003eIsolation and purification of nuclei from tissues and cultured cells are common lab practices. Isolated nuclei, especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and apoptosis research. The traditional method for the isolation of nuclei is relatively simple. However, nucleus aggregation and formation of a high percentage of doublets are frequently encountered problems. This kit is specially designed to address these issues. The protocol is simple, rapid, and very effective. A high percentage of the well-separated single nucleus can be obtained in about 30 min. In comparison to the traditional method, the kit requires less starting material and has a larger range of sample sizes (1-30 mg). The buffers contain a proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not desired, a detergent-free nuclei isolation kit is also available under cat# NI-024.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180558042,"sku":"SN-047","price":1104.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/SN-047_35a04081-2a8b-4d46-b967-55d1d5d9227c.webp?v=1758812136"},{"product_id":"s46","title":"MinuteTM Rubisco Depletion Kit","description":"\u003cp\u003ePlants perceive signals from the environment through leaves. One of the major goals of the scientific community is to dissect the components of signaling cascades by analyzing the leaf proteome. However, the major bottleneck is presence of a high-abundant protein —Rubisco, which accounts for majority of leaf protein ranging from 40-80%. High level rubisco masks the detection and identification of low abundant proteins by LC-MS\/MS-based method. A specific IgY conjugated resin is available commercially for depletion of rubisco from extracted total protein but only small amount of protein can be handled and it is very costly. Other methods have been described in the literatures but are usually tedious and time consuming. The MinuteTM Rubisco Depletion Kit is designed to overcome these disadvantages by using a specialized filter cartridge coupled with proprietary rubisco depletion buffer. The same, if not better, results can be obtained with a much easier and straighter forward protocol in about 1h. The rubisco-depleted total protein can be used for many downstream applications such as SDS-PAGE, 2DE and LC MS\/MS analysis.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"20 Preps","offer_id":46116180590810,"sku":"RD-046","price":1002.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/RD-046_9a16bd1a-8dbc-4a23-8543-4f03d6787233.jpg?v=1758812140"},{"product_id":"s47","title":"Minute TM High Efficiency Protein Precipitation kit","description":"\u003cp\u003eProtein precipitation stands out as a clear strategy for concentrating proteins and eliminating interfering substances present in protein samples, such as salts, lipids, and other elements that might disrupt subsequent applications. Among the widely employed techniques, trichloroacetic acid (TCA) precipitation emerges as a straightforward and efficient method. However, proteins precipitated through the TCA method typically undergo denaturation, often resulting in reduced solubility.\u003c\/p\u003e\n\n\u003cp\u003eA notable drawback of the conventional TCA\/acetone precipitation approach is its diminished efficacy when dealing with samples featuring low protein concentrations. Higher protein concentration samples are precipitated much more efficiently compared to those with lower protein concentrations. In response to these limitations, we have innovated a high-efficiency protein precipitation kit, a refinement of the traditional TCA method. This kit is characterized by its simplicity, rapidity, and heightened effectiveness. It enables the effective precipitation and concentration of proteins at low concentrations in less than 30 minutes.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"30 ml","offer_id":46116180623578,"sku":"WA-006","price":581.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/WA-006_080a87ad-3182-4a08-a92e-a0cb9afb3357.webp?v=1758812142"},{"product_id":"s48","title":"MinuteTM Denaturing Protein Solubilization Reagent","description":"\u003cp\u003eThis reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are denatured due to the presence of an anionic detergent (SDS). (Cat. No. WA-012, Invent Biotechnologies).\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"10 ml","offer_id":46116180656346,"sku":"WA-009","price":403.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/WA-009_303ea6a2-d37a-4a18-9349-add81824e899.webp?v=1758812146"},{"product_id":"s49","title":"MinuteTM Non-Denatured Protein Solubilization Reagent","description":"\u003cp\u003eThis reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are non-denatured and can be used for ELISA, immunoprecipitation\/Co-IP, enzymatic activity determination and other applications.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"10 ml","offer_id":46116180689114,"sku":"WA-010","price":403.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/WA-010_ed480822-00d8-4717-843d-ed4b030722e1.webp?v=1758812147"},{"product_id":"s50","title":"MinuteTM Protein Solubilization Reagent for MS","description":"\u003cp\u003eThis reagent is formulated for dissolving protein and organelle pellets using Minute™ kits from Invent Biotechnologies or similar products of other manufacturers. Solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"2 ml","offer_id":46116180721882,"sku":"WA-011","price":403.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/WA-011_1dd6d9a0-6e80-4623-bdc6-11a3bc534993.webp?v=1758812149"},{"product_id":"s51","title":"MinuteTM SDS-Remover","description":"\u003cp\u003eSodium dodecyl sulfate is one of the most commonly used detergents for biological research. However, the presence of SDS in the sample could interfere with downstream applications. For instance, SDS must be reduced to below a certain concentration before trypsin digestion of protein can be performed effectively. The presence of SDS in a protein sample could interfere with, and needs to be removed prior to, the analysis. The presence of SDS in protein samples also interferes with antigen-antibody binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary formulated reagent is designed for fast and easy removal of SDS in solution by precipitation. The efficacy is superior to SDS-removal column and TCA precipitation with minimum protein loss (\u0026lt;20%). Due to the small volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.\u003c\/p\u003e","brand":"Invent Biotechnologies","offers":[{"title":"10 ml","offer_id":46116180754650,"sku":"WA-012","price":437.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0716\/2553\/9802\/files\/WA-012_2932195f-3975-4a8f-9f76-82614b53f526.webp?v=1758812151"}],"url":"https:\/\/q2ub.com\/collections\/protein-biology.oembed?page=25","provider":"Q2UB","version":"1.0","type":"link"}